Inhibitory synapses in the central nervous system rely on scaffold proteins like gephyrin to properly anchor GABA and glycine receptors, and disruptions in this system are linked to neurological disorders such as epileptic encephalopathy. This project investigates how different gephyrin splice variants (especially those involving C4 exons) influence the formation and plasticity of inhibitory synapses using genetic, biochemical, and electrophysiological approaches. The findings aim to determine whether distinct gephyrin isoforms create functionally diverse synapses, potentially explaining differences in vulnerability to neurological diseases.